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renal proximal tubule epithelial cell rptec immortalized cell line  (ATCC)


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    ATCC renal proximal tubule epithelial cell rptec immortalized cell line
    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells <t>(RPTEC)</t> with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.
    Renal Proximal Tubule Epithelial Cell Rptec Immortalized Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/renal+proximal+tubule+epithelial+cell+line/Primary+Renal+Proximal+Tubule+Epithelial+Cells%3B+Normal%2C+Human/pmc13078729-89-2-11
    Average 99 stars, based on 751 article reviews
    renal proximal tubule epithelial cell rptec immortalized cell line - by Bioz Stars, 2026-09
    99/100 stars

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    1) Product Images from "The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5"

    Article Title: The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5

    Journal: Comprehensive Physiology

    doi: 10.1002/cph4.70147

    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.
    Figure Legend Snippet: Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Techniques Used: Recombinant, Gene Expression, Expressing

    Related Articles

    Cell Culture:

    Article Title: Pivotal role of AhR protein in the therapeutic efficacy of HuangKui capsule against calcium oxalate-induced kidney damage: An integrated study of network pharmacology and experimental verification.
    Article Snippet: Kidney stone are a frequently-occurring disease that may lead to severe renal impairment and eventual renal malfunction.. The aryl hydrocarbon receptor (AhR) is a cytoplasmic receptor and transcription factor that belongs to the family of basic helix-loop-helix transcription factors, which plays an important role in the pathogenesis of kidney stones.. The HuangKui Capsule (HKC) is a Traditional Chinese Medicine used for managing chronic glomerulonephritis.

    Article Title: Berberine Attenuates Pyroptosis in Renal Tubular Epithelial Cells by Activating the FOXO3a/ARC Pathway.
    Article Snippet: .. The human renal proximal tubule epithelial cell line (HK- 2) was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Logan, UT, USA) supplemented with 10% fetal bovine serum and 1% penicillin- streptomycin. ..

    Article Title: Caspase-3 Promotes Diabetic Kidney Disease Through Gasdermin E-Mediated Progression to Secondary Necrosis During Apoptosis
    Article Snippet: .. We cultured a human renal proximal tubule epithelial cell line (HK-2 cells; ATCC ® CRL-2190TM) in DMEM/F12 supplemented with 100U/mL penicillin and streptomycin and 10% FBS (#1009-1148, Gibco) at 37°C in a 5% CO 2 environment. .. We prepared DMEM/F12 medium with DMEM (#11885-084, Gibco), containing 1g/l glucose, and F12 (#11765-054, Gibco) at a 1:1 ratio.

    Modification:

    Article Title: Berberine Attenuates Pyroptosis in Renal Tubular Epithelial Cells by Activating the FOXO3a/ARC Pathway.
    Article Snippet: .. The human renal proximal tubule epithelial cell line (HK- 2) was obtained from the American Type Culture Collection (ATCC) and cultured in Dulbecco's Modified Eagle Medium (DMEM; Gibco, Logan, UT, USA) supplemented with 10% fetal bovine serum and 1% penicillin- streptomycin. ..



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    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Journal: Comprehensive Physiology

    Article Title: The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5

    doi: 10.1002/cph4.70147

    Figure Lengend Snippet: Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Article Snippet: The human Renal Proximal Tubule Epithelial Cell (RPTEC) immortalized cell line (ATCC, PCS-400–010) was cultured in complete growth medium consisting of the base medium containing DMEM: F12 Medium (ATCC 30–2006) and the RPTEC Growth Kit components (ATCC ACS-4007) consisting of 5 pM triiodo-L-thyronine, 10 ng/mL recombinant human EGF, 3.5 μg/mL ascorbic acid, 5.0 μg/mL human transferrin, 5.0 μg/mL insulin, 25 ng/mL prostaglandin E 1 , 25 ng/mL hydrocortisone, 8.65 ng/mL sodium selenite and 1.2 mg/mL sodium bicarbonate.

    Techniques: Recombinant, Gene Expression, Expressing